How long pcr product can be stored

Most thermal cyclers in the market heat and cool using Peltier or thermoelectric cooling elements. While this is a great technology, thermoelectric elements are notorious for failing under heavy use and are extremely fragile. They are especially prone to breakage during transportation. Peltier elements have a finite lifespan, and every moment a thermocycler is left on counts toward that span. Cold holds, or holds at 4 to 10 degrees Celsius, can cause air moisture to condense on the metal block that holds PCR samples.

This condensation can damage the block over the long run. If water finds its way to the underside of the block, it may drip into the electrical components, causing electrical malfunction.

After all, the DNA double helix evolved to preserve our genetic information. Paleontologists amplify ancient DNA from bones deposited 50, years ago, and forensic scientists routinely collect DNA evidence from messy crime scenes. If we can study DNA from samples that have been in the environment for hundreds and even thousands of years, it is to be expected that our PCR bands will survive a night at room temperature. Shop miniPCR. Life Science. This condensation can actually cause damage to the block overtime, significantly reducing the lifespan of the machine.

Not to mention, by simply setting your temperature that low, you are already requiring more work from your equipment. Jennifer Joiner, Molecular Staging Inc. CSR News, 2. John G. Lasken RS, Egholm M. While this is a great technology, thermoelectric elements are notorious for failing under heavy use and are fragile, especially prone to breakage during transportation. Peltier elements have a finite lifetime and every moment a thermocycler is left on counts towards that lifespan.

We will discuss advantages of NOT using Peltier in an upcoming post. One other thing. Holds at degrees can cause air moisture to condense on the metal block that holds PCR samples. This condensation can damage the block over the long run, and if water finds its way to the underside of the block, it may drip into the electrical components causing electrical malfunction. After all, the DNA double helix has evolved to preserve our genetic information. Paleontologists amplify ancient DNA from bones deposited 50, years ago, and forensic scientists routinely collect DNA evidence from messy crime scenes.

If we can study DNA from samples that have been out in the environment for hundreds and even thousands of years, it is to be expected that our PCR bands will survive one night at room temperature. Questions about PCR? BioCoder is the self-described newsletter of the biological revolution. And miniPCR is the poster child of the Fall